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Figure 3 | Virology Journal

Figure 3

From: Expression of HIV receptors, alternate receptors and co-receptors on tonsillar epithelium: implications for HIV binding and primary oral infection

Figure 3

Double label immunofluorescence detection of CXCR4 or CCR5 co-receptor positive T cells at tonsillar epithelial surfaces. Thin sections were incubated with primary antibodies and species-specific fluorescently conjugated secondary antibodies as indicated. All sections were stained with DAPI (blue) to identify cell nuclei. a: CD3 (green) + CCR5 (red): DAPI overlay; b: CD3 (green) + CCR5 (red): no DAPI overlay; c: enlargement of the region enclosed in the box in b; d: CD3 (green) + CXCR4 (red): DAPI overlay; e: CD3 (green) + CXCR4 (red): no DAPI overlay; f: enlargement of the region enclosed in the box in e. Cells that are positive for both markers appear yellow; a-c depict stratified squamous epithelium, d-f depict reticulated cryptal epithelium. Original magnification a, b, d, e: ×100.

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