Predicting the subcellular localization of viral proteins within a mammalian host cell

Table 3 Subcellular localization prediction of cytomegalovirus proteins

Gene	SwissProt Accession	PSLT predictions^a	Literature annotations	Localization References	Closest human homologue^b	BLAST e-value
gp34	P16809	PM	PM	[6]	-	> 0.1
UL111A	P17150	Secreted	Secreted	[36]	interleukin 10 (NP_000563)	2E-9
UL114	P16769	Nuclear	Nuclear	[66]	uracil-DNA glycosylase (NP_550433)	5E-39
UL118/119	P16739	PM	PM	[6]	-	> 0.1
UL18	P08560	PM/secreted	PM	[67]	MHC class Ib antigen (NP_005507)	4E-16
UL33	P16849	PM	PM, endosomes, secretory pathway, perinuclear	[34,68]	chemokine receptor 1 (NP_001286)	3E-21
UL48	P16785	Cytosolic and ER	ER, cytosolic, vacuolar	[69,70]	spen homolog, transcriptional regulator (NP_055816)	0.082
UL54	P08546	Nuclear	Nuclear	[71]	polymerase delta 1 (NP_002682)	3E-50
UL78	P16751	PM	PM	[34]	somatostatin receptor 3 (NP_001042)	0.006
UL97	P16788	PM, cytosolic	Golgi, nuclear and cytosolic	[72]	-	> 0.1
US10	P09728	PM	ER	[33]	-	> 0.1
US11	P09727	PM	ER	[4]	-	> 0.1
US2	P09713	PM	ER and cytosolic	[5]	-	> 0.1
US27	P09703	PM	PM, endosomes, secretory pathway, perinuclear	[34,68]	chemokine receptor 1 (NP_001328)	7E-31
US28	P69332	PM	PM, endosomes	[34,68]	chemokine receptor 1 (NP_001328)	2E-55
US3	P09712	PM	ER	[73,74]	-	> 0.1
US6	P14334	PM	ER	[75]	-	> 0.1
US7	P09731	PM	ER	[33]	-	> 0.1
US8	P09730	PM	Golgi	[33]	-	> 0.1
US9	P09729	Secreted	ER	[33]	-	> 0.1

^a In the case of multi-compartmental proteins (proteins that are predicted with high probability to be present in more than one compartment), the two most likely compartments were retained by PSLT. PM: plasma membrane; ER: endoplasmic reticulum.
^b The closest human homologue was determined by using BLAST [38] against the NCBI human RefSeq dataset. We do not report a homologue when the BLAST e-value exceeds 0.1.

ISSN: 1743-422X