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Table 2 Inter assay variability of the newly developed qPCR assay

From: Application of real-time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia

 

DNA (copies per mL) on different days

Sample ID

Day 1

Day 2

Day 3

Average

SD

CV (%)

MVA300

2.7 × 106

1.4 × 106

2.7 × 106

2.3 × 106

0.19

3.0

MVA307

2.1 × 109

4.2 × 109

1.5 × 109

2.6 × 106

0.26

2.70

MVA313

1.4 x108

9.8 × 107

1.3 × 108

1.2 × 108

0.09

1.10

MVA315

1.3 × 106

3.3 × 106

2.0 x106

2.2 × 106

0.01

1.20

T4040

5.6 × 103

7.2 × 103

4.3 × 103

5.7 × 103

0.23

0.62

Negative control

DNA not detected

DNA not detected

DNA not detected

DNA not detected

  
  1. The six samples represented in the table include five positive samples (MVA 300, MVA 307, MVA 313, MVA 315) and one negative sample (negative for HBsAg). The samples were tested on three different days.
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Virology Journal

ISSN: 1743-422X