Figure 3

A-MLV binding to large rafts is independent of extraction of plasma membrane cholesterol. NIH3T3 cells were treated with 10 mM MBCD, washed, and incubated with GagYFP A-MLV (green) for 30 min. Subsequently, the cells were washed, fixed, and stained for GM1 using fluorescently labeled CTX (red). Images were taken using confocal microscopy.