Figure 2

Large rafts are present in NIH3T3 cells independent of A-MLV binding. A), and B) NIH3T3 cells were incubated with GagYFP A-MLV (green) for 30 min, fixed, and stained for GM1 with fluorescently labeled CTX (red). Clusters of viral particles bound to large rafts are labelled with arrows in B). C), and D) NIH3T3 were incubated with VSV (green) for 30 min, fixed, and stained for GM1 with fluorescently labeled CTX (red). All images were taken using confocal microscopy. A) and C) are merged images, B) and D) show only GagYFP A-MLV or GagYFP VSV particles from A) and C), respectively.