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Table 2 Sequence substitution of the 3' sequences of pS2CAT96

From: The 3' sequences required for incorporation of an engineered ssRNA into the Reovirus genome

cDNA Clone Length of 5' S2 sequence Length of 3' S2 sequence Substituted Sequence CAT Activity [pg/ml] ssRNA detected dsRNA detected Engineered RNA incorporated into infectious virus
pS2CAT3m 96 98 none 64 + + +
pS2CATsub1 96 98 98-89 64 + - -
pS2CATsub2 96 98 88-79 64 + - -
pS2CATsub3 96 98 78-69 32 + + +
pS2CATsub4 96 98 68-59 32 + - -
pS2CATsub5 96 98 58-49 64 + + +
pS2CATsub6 96 98 48-39 64 + + +
pS2CATsub7 96 98 38-29 32 + + +
pS2CATsub8 96 98 28-19 32 + - -
pS2CATsub9 96 98 18-9 64 + - -
pS2CATsub10 96 98 8-1 32 + - -
pS2CATsub11 96 98 98-94 64 + - -
pS2CATsub12 96 98 93-89 64 + - -
pS2CATsub13 96 98 88-84 64 + - -
pS2CATsub14 96 98 83-79 64 + + +
pS2CATsub15 96 98 68-64 64 + - -
pS2CATsub16 96 98 63-59 64 + - -
pS2CATsub17 96 98 28-24 32 + - -
pS2CATsub18 96 98 23-19 64 + - -
pS2CATsub19 96 98 18-14 64 + - -
pS2CATsub20 96 98 13-9 32 + - -
pS2CATsub21 96 98 8-4 64 + - -