The 3' sequences required for incorporation of an engineered ssRNA into the Reovirus genome

Table 2 Sequence substitution of the 3' sequences of pS2CAT96

cDNA Clone	Length of 5' S2 sequence	Length of 3' S2 sequence	Substituted Sequence	CAT Activity [pg/ml]	ssRNA detected	dsRNA detected	Engineered RNA incorporated into infectious virus
pS2CAT3m	96	98	none	64	+	+	+
pS2CATsub1	96	98	98-89	64	+	-	-
pS2CATsub2	96	98	88-79	64	+	-	-
pS2CATsub3	96	98	78-69	32	+	+	+
pS2CATsub4	96	98	68-59	32	+	-	-
pS2CATsub5	96	98	58-49	64	+	+	+
pS2CATsub6	96	98	48-39	64	+	+	+
pS2CATsub7	96	98	38-29	32	+	+	+
pS2CATsub8	96	98	28-19	32	+	-	-
pS2CATsub9	96	98	18-9	64	+	-	-
pS2CATsub10	96	98	8-1	32	+	-	-
pS2CATsub11	96	98	98-94	64	+	-	-
pS2CATsub12	96	98	93-89	64	+	-	-
pS2CATsub13	96	98	88-84	64	+	-	-
pS2CATsub14	96	98	83-79	64	+	+	+
pS2CATsub15	96	98	68-64	64	+	-	-
pS2CATsub16	96	98	63-59	64	+	-	-
pS2CATsub17	96	98	28-24	32	+	-	-
pS2CATsub18	96	98	23-19	64	+	-	-
pS2CATsub19	96	98	18-14	64	+	-	-
pS2CATsub20	96	98	13-9	32	+	-	-
pS2CATsub21	96	98	8-4	64	+	-	-

ISSN: 1743-422X