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Figure 7 | Virology Journal

Figure 7

From: Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinant

Figure 7

PKR mediates phosphorylation of eIF-2α and inhibition of translation caused by the expression of HCV polyprotein. A: Immunoblot analysis of total cell protein lysates prepared from PKR knockout (PKR-/-) and PKR WT (PKR+/+) cells infected with the parental (VT7) or the recombinant VT7-HCV7.9 viruses in the presence (+) of IPTG for 24 h. The blot was first probed with a polyclonal antibody that recognized phospho-eIF-2α-S51 protein, stripped twice, and reprobed with a polyclonal antibody that recognizes total eIF-2α protein and a monoclonal antibody against β-actin. B: Wild type and PKR-/- cell lines infected with VT7-HCV7.9 in the presence (+) or absence (-) of IPTG were metabolically labelled with 35S-Met-Cys Promix (50 μCi/mL) at 16 h.p.i, fractionated by SDS-PAGE and analysed by autoradiography. The recombinant VV-PKR virus was used as a control. U: uninfected cells.

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Virology Journal

ISSN: 1743-422X