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Figure 10 | Virology Journal

Figure 10

From: Involvement of PKR and RNase L in translational control and induction of apoptosis after Hepatitis C polyprotein expression from a Vaccinia virus recombinant

Figure 10

Expression of HCV polyprotein from VV inhibits cellular and viral protein synthesis in RL+/+ and in RL-/- infected cells. A: RL+/+ and RL-/- cells infected with VT7-HCV7.9 in the presence (+) or absence (-) of IPTG were metabolically labelled with 35S-Met-Cys Promix (50 μCi/mL) at 8 h.p.i, fractionated by SDS-PAGE and analysed by autoradiography. U: uninfected cells. B: Immunoblot analysis of total cell protein lysates prepared from RL+/+ and RL-/- cells infected with VT7-HCV7.9 in the presence (+) or absence (-) of IPTG for 8 h. The blot was first probed with a polyclonal antibody that recognized phospho-eIF-2α-S51 protein, stripped and reprobed with a polyclonal antibody that recognizes total eIF-2α protein.

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Virology Journal

ISSN: 1743-422X