Figure 3
From: Human enterovirus 71 subgenotype B3 lacks coxsackievirus A16-like neurovirulence in mice infection
EV-71 and CV-A16 infections of newborn mice. One-day old newborn mice were intracerebrally inoculated with 1 × 103 PFU virus per mouse and monitored daily. CV-A16-infected mice had floppy tails on day two post-inoculation (a) and hind leg paralysis beginning on day three post-inoculation (arrow, b). Mice with swollen limbs were noted in EV-71 B3 virus infection (arrow, c) and the EV-71 B3-infected mice had significantly reduced body weight gain in comparison to the mock-infected mice (d, V = B3-infected mouse, C = mock-infected mouse). Mice with floppy tails, swollen limbs and paralysis (e) and death (f) were recorded. The weight gain of the surviving mice was also determined (g).