Figure 1From: The SARS Coronavirus S Glycoprotein Receptor Binding Domain: Fine Mapping and Functional CharacterizationExpression and binding of soluble S fragments containing the RBD. A) Soluble S proteins concentrated using Ni-NTA agarose beads from the supernatants of 293 cells transfected with various constructs were run, blotted onto a nitrocellulose membrane and detected with anti-c-myc epitope antibody. B) Cell binding assay data using supernatants described above, shown as a percentage of the reading of S272–537 that has been used in this experiment as a positive control.Back to article page