Serum anti-HSV-1 antibody responses. (a) Serum anti-HSV-1 antibody responses of BALB/c scid (N = 8) and immunocompetent BALB/c (N = 8) mice in the Latent Protocol. The mice were grafted with ganglia containing latent virus and randomly chosen mice were bled on days 21, 45, and 65 after transplantation. The corrected optical density readings indicate that the BALB/c scid mice did not produce IgG antibody, whereas the immunocompetent BALB/c mice all had serum IgG anti-HSV-1 antibody on days 45 and 65 after transplantation. (b) Serum antibody responses of BALB/c mice receiving acutely infected ganglia (N = 4) or ganglia containing reactivating virus (N = 4). The optical density readings indicate that the immunocompetent mice in the Acute Protocol and the Reactivation Protocol developed serum anti-HSV-1 antibody titers by day 21 after grafting and that this antibody continued to be present on days 45 and 65 after transplantation. At each of the three time points, symbols representing one mouse each are spread out to avoid concealment by overlap.