Figure 8
From: Intracellular localization of Crimean-Congo Hemorrhagic Fever (CCHF) virus glycoproteins
Subcellular fractionation studies of expressed GFP-CCHFV G fusion proteins. Expression plasmids for GFP-CCHFV glycoprotein fusion protein were transfected into BHK-21 cells and used for a subcellular fractionation study to determine if fusion proteins containing different parts from the CCHFV GN cytoplasmic domain are soluble or membrane associated. A GFP-specific antibody was used for the immunoblot. Representative results from three constructs are shown. M: membrane fraction, S: soluble fraction.