Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysis

Table 1 Clinical and virological characteristics of the 20 HALT-C patients.

Patient	Age	Gender	Genotype	Serum HCV RNA at W00 (log₁₀)	Diversity		Complexity
					Taq	HF-2	Taq	HF-2
1	52	M	1a	6.55	0.9953	1.0000	7	1
2	54	F	1b	6.49	0.9905	1.0000	5	1
3	51	M	1a/b	6.99	0.9958	1.0000	5	1
4	42	M	1a	6.81	0.9877	0.9894	6	2
5	56	M	1a	7.13	0.9979	1.0000	4	1
6	42	M	1	6.55	0.9903	0.9912	8	6
7	73	M	1b	6.78	0.9892	0.9877	7	5
8	45	M	1	6.37	0.9193	0.9805	7	6
9	53	M	1a	7.06	0.9954	1.0000	2	1
10	47	M	1b	6.35	0.9845	0.9994	4	3
11	19	M	1b	5.72	0.9964	0.9994	2	2
12	49	M	1a	7.06	0.9979	0.9986	3	3
13	42	F	1b	6.26	0.9993	0.9781	2	2
14	42	M	1a	7.30	0.9810	0.9685	8	9
15	61	M	1b	5.85	0.9858	0.9624	4	5
16	54	M	1b	6.93	0.9047	0.9409	6	9
17	45	F	1a	6.72	0.9962	0.9917	2	5
18	47	F	1a	6.14	0.9966	0.9984	4	2
19	50	M	1a	6.37	0.9962	0.9968	4	3
20	53	M	1a	5.81	0.989	0.9943	6	5
AVG	48.85			6.56	0.9845	0.9889	4.8	3.6

HCV genotypes were determined by INNO-LiPA. HCV RNA was quantified with the Roche COBAS Monitor assay, and is expressed as log₁₀ IU/mL. QS heterogeneity was assessed using the clonal frequency analysis (CFA) technique. Diversity scores represent the average heteroduplex mobility ratios (HMR) for either Taq or HF-2 enzymes. Complexity scores represent the total number of distinct gel shift variants analyzed by CFA. W00 represents the week 0 or baseline sample. AVG represents average.

ISSN: 1743-422X