Effect of UNG on Standard curves for competitor RNA. Linear regression analysis for each standard curve was performed within the analysis software (Stratagene Mx4000 version 4.00). Symbols represent means for samples analyzed in triplicate. Addition of 0.25 units UNG per reaction followed by incubation at 30°C for 20 min prior to RT-PCR (●), no addition of UNG but incubation at 30°C for 20 min prior to RT-PCR (○), no addition of UNG and no incubation prior to RT-PCR (▼). CT, cycle threshold. dRn, baseline-corrected normalized fluorescence.