2D gel electrophoresis image of detergent extracts from PS Clone D cell monolayer. The first dimension was run on linear 7 cm IPG strip, pH 3-10. The second dimension was 10% SDS PAGE. The gel was stained with coomasie brilliant blue and spots were picked and subjected to in-gel trypsin digestion. The major spots identified by MALDI TOF are labelled. Circled spots were reactive in the 2D VOPBA. (a) NaDOC-extract (b) βOG-extract (c) NaDOC-extract after removal of βOG-soluble proteins.