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Figure 4 | Virology Journal

Figure 4

From: Origin-independent plasmid replication occurs in vaccinia virus cytoplasmic factories and requires all five known poxvirus replication factors

Figure 4

Intracellular localization of replicated plasmid containing tandem lac O repeats using a lac I-GFP fusion protein. HeLa cells were transfected with pSV2-dhfr-8.32 containing tandem lac O repeats (top 2 panels) or p716 control plasmid (bottom 2 panels) and infected with vGFP-lac I. At 12 h after infection, cells were fixed, permeabilized, and stained with antibody to VAC RNA polymerase (anti-RNAP), followed by Alexa 594-conjugated goat anti-rabbit IgG. Cells were then stained with Hoechst dye and analyzed by confocal microscopy. Blue, Hoechst; red, Alexa 594; and green, GFP fluorescence.

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Virology Journal

ISSN: 1743-422X