Table 1 List of the oligonucleotide primers used in this study for amplification of cassava mosaic geminiviruses from Tanzania (anfl = near-full length, ps = partial sequence)
Primer name | Nucleotide sequence (5'→3') | Begomovirus isolate | DNA component |
|---|---|---|---|
UGT-F | TCGTCTAGAACAATACTGATCGGTCTCC | EACMV-KE-[TZT] | DNA-A fla |
UGT-R | CGGTCTAGAAGGTGATAGCCGAACCGGGA | EACMV-KE-[TZT] | DNA-A fl |
3T-F | ACGTCTAGAACAATACTGATCGGTCTC | EACMV-TZ-[YV] | DNA-A fl |
3T-R | GTGCTCTAGAAGGTGATAGCCGAACCGGGA | EACMV-TZ-[YV] | DNA-A fl |
TZ1B-F | GCGCGGAATCACTTGTGAAGCAGTCGT | EACMCV-[TZ1] | DNA-B fl |
TZ1B-R | GCCGGGATTCGGTGAGTGGTTTACATCAC | EACMCV-[TZ1] | DNA-B fl |
EAB555/F | TACATCGGCCTTTGAGTCGCATGG | CMGs | BC1/CR |
EAB555/R | CTTATTAACGCCTATATAAACACC | CMGs | BC1/CR |
UNI/F | KSGGGTCGACGTCATCAATGACGTTRTAC | CMGs | DNA-A nfl |
UNI/R | AARGAATTCATKGGGGCCCARARRGACTGGC | CMGs | DNA-A nfl |
AT-F | GTGACGAAGATTGCATTCT | ACMV-[TZ] | DNA-A ps |
AT-R | AATAGTATTGTCATAGAAG | ACMV-[TZ] | DNA-A ps |
ATZ1-F | TAAGAAGATGGTGGGAATCC | EACMCV-[TZ1] | DNA-A ps |
ATZ-R | CGATCAGTATTGTTCTGGAAC | EACMCV-[TZ1] | DNA-A ps |
TZ7-F | TGGTGGGAATCCCACCTT | EACMCV-[TZ7] | DNA-A ps |
TZ7-R | GTATTGTTATGGAAGGTGATA | EACMCV-[TZ7] | DNA-A ps |
TZM-F | TATATGATGATGTTGGTC | EACMV-UG2Svr-[TZ10] | DNA-A ps |
TZ10-R | TAGAAGGTGATAGCCGTA | EACMV-UG2Svr-[TZ10] | DNA-A ps |
TZM-F | TATATGATGATGTTGGTC | EACMV-KE-[TZM] | DNA-A ps |
TZM-R | TAGAAGGTGATAGCCGAAC | EACMV-KE-TZM] | DNA-A ps |