Synergistic inhibition of human cytomegalovirus replication by interferon-alpha/beta and interferon-gamma

Table 1 Effect of IFN-α, IFN-β and/or IFN-γ on HCMV plaque formation

Treatment	IU/ml^a	Log (mean no. of plaques) ± sem	Fold-inhibition^c
Vehicle	---	3.34 ± 0.02^b	---
IFN-α	100	2.38 ± 0.01*	9
IFN-α	200	2.30 ± 0.01*	11
IFN-β	100	1.77 ± 0.05*	37
IFN-β	200	1.77 ± 0.02*	37
IFN-γ	100	1.88 ± 0.03*	29
IFN-γ	200	1.85 ± 0.02*	30
IFN-α and IFN-β	100	1.95 ± 0.04*	25
IFN-α and IFN-γ	100	1.13 ± 0.09*	164
IFN-β and IFN-γ	100	0.52 ± 0.05*	662
IFN-α, IFN-β and IFN-γ	100	0.66 ± 0.15*	512

^aHFFs were treated with either 100 or 200 IU/ml each of IFN-α, IFN-β or IFN-γ (separately or in combination).
^bMean ± sem of viral plaque formation on HFFs observed in 3 replicates per group. Cultures were infected with 2000 PFU/well of Towne-GFP, and plaque numbers were determined 14 d p.i. by fluorescent microscopy.
^cFold-inhibition was calculated as: 10^{([log plaques / PFU in vehicle-treated] - [log plaques / PFU in IFN-treated])}
* Significant reduction in plaque numbers of IFN-treated groups as compared to vehicle-treated groups is denoted by a single asterisk (P < 0.001, one-way ANOVA and Tukey's post hoc t test).

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