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Figure 4 | Virology Journal

Figure 4

From: Cloning and expression of N22 region of Torque Teno virus (TTV) genome and use of peptide in developing immunoassay for TTV antibodies

Figure 4

Dot blot assay using human sera. A). Dilutions of sera in PBS were used as primary antibody. Positive control indicates the use of anti-His antibody to bind with antigen pre-coated on NC membrane. Serum without TTV-DNA and anti-His was used as negative control. Strip 1: PBS only; Strip 2: Negative control; Strip 3: Positive control; Strip 4: 1:500 sera dilution; Strip 5: 1:1000 sera dilution; Strip 6: 1:2000 sera dilution; Strip 7: 1:3000 sera dilution; Strip 8: 1:5000 sera dilution. B). Dot blot assay using sera positive and negative for anti-TTV antibodies. Strips 1–4: Anti-TTV negative sera; Strips 5–8: Anti-TTV positive sera.

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Virology Journal

ISSN: 1743-422X