Table 2 Primers used to amplify the CTNCEC25 structural protein gene sequence
Primer | Sequence (5′ → 3′) | Target gene |
|---|---|---|
N-F | ACGCTTAACAACCAAATCAAAG | N |
N-R | TTGACGAAGATCTTGCTCAT | |
P-F | CGTACTCTAGTGACTCGTAA | P |
P-R | ATCTTGCGTAGAAAGTTCAT | |
M-F | GGTGGGTTGCTCTGGCTAA | M |
M-R | AGGCAGAAGACACCGTTATT | |
G-F | ATACGGGCTTAACTCCAACCT | G |
G-R | GCTCGGCCTCTGACTCAAT | |
L1-F | AGGGTCATATCTTCATGGGA | L |
L1-R | TCGCTCGCCAAGCACTCC | |
L2-F | TGGAAATTCAGGTTATGAAGTC | |
L2-R | ACAGGGCTTTCCTGATCGCATC | |
L3-F | CGAGGTAACATCTTGGTGCC | |
L3-R | TGAGTCATGTATCGCGACCA | |
L4-F | CTCAGGGGCTTCTATATT | |
L4-R | ATCATCTCCTCCACTCAT | |
L5-F | GTGGGTTTGTTCCGCTC | |
L5-R | AGAGGTTCTGATTTGAGA |