Oncolytic therapy of a recombinant Newcastle disease virus D90 strain for lung cancer

Table 1 Primer sequences used in the study

Primer	Primer sequence (5′-3′)^a
D90-F4-F	ATAAACTAGTACGCGT GGTtTCAGGCTTATATGCAG
D90-F4-R	ATGCCATGCCGACCCACCAAACAGAGATTTGGTGAATG
D90-F1-F	AATACGACTCACTATAGGACCAAACAGAGAATCTGTGAG
D90-F1-R	ACGCGTACTAGTTTATAA AGTGCCTGGATGGTCAGCTG
D90-F2-F	CATCCAGGCACTTTATAA TTTAGCTGGTGGCAATATG
D90-F2-R	TAATACGCGTACTAGT AAGGGAACGATCCTAAATTC
D90-F3-F	TCGTTCCCTTACTAGT TGAGATCCTCAAGGATGATAG
D90-F3-R	GCCTGAAACCACGCGTCGAGTGCAAGAGACTAACAATC
EGFP-F	GCGTCACACGGAATCCCGCGGAGTTAGAAAAAATACGGGTAGAACGCCACCatggtgagcaagggcgaggagctg
EGFP-R	CGCGAGGGGGGGCCCCCGCGGttacttgtacagctcgtccatgccg
NP-F-Kozak	TATTGAATTC GCCACCATGTCGTCTGTTTTCGACGAATAC
NP-R	TATATCTAGA TCAGTACCCCCAGTCAGTGTC
P-F-Kozak	TATTGAATTC GCCACCATGGCCACTTTTACAGATGC
P-R	TATATCTAGA TCAACCATTtAGCGCAAGGCG
L-F-Kozak	TATTGTCGAC GCCACCATGGCGGGCTCCGGTCCCGAAAG
L-R	TATAGCGGCCGC TTAAGAGTCATTATTACTGTAATATC

^aThe restriction enzyme sites within the primers were marked in bold type. Nucleotides shown in lower case letters represent the sequence from EGFP.

ISSN: 1743-422X