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Table 2 PCR results using five different nested assays

From: Molecular characterisation of human hepatitis E virus from Italy: comparative analysis of five reverse transcription-PCR assays

Patient code Real-Time PCRa RT-PCR/nestedPCR Country of Originb
Method A (ORF1, MTase) Method B (ORF1, RdRp) Method C (ORF2, capsid) Method D (ORF2, capsid) Method E (ORF2/ORF3)
8 + + + Genotype 1 Genotype 1 Genotype 1a Genotype 1a Genotype 1 Italy (India)
10 + -   - - - Ethiopia
12 + + + -   - - Genotype 1 India
16 + + Genotype 1   Genotype 1a Genotype 1a Genotype 1 Bangladesh
19 + + -   - - - Bangladesh
22 + + - Genotype 1 Genotype 1a Genotype 1a Genotype 1 Italy (India)
29 + -   - - - Bangladesh
33 + -   - - - Italy
34 + -   - - - Italy
43 + + Genotype 1   - - - Bangladesh
47 + + -   - - - Bangladesh
48 + + - Genotype 1 - - - Bangladesh
51 + + -   - - - Italy
52 + Genotype 1   Genotype 1a - - Bangladesh
55 + + Genotype 1   - - - India
56 + + -   - - - Bangladesh
58 + + Genotype 1   - - - Italy (Thailand)
61 + + -   - - - Italy (India)
63 + + + -   - - - Albania
69 + Genotype 3   - - Genotype 3 Italy
71 + -   - - - Bangladesh
81 + -   - - - Italy
86 + -   - - - Italy
92 + Genotype 3   - - - Italy
  1. a+ : 250 < copies/mL < 2,500; + + : 2,500 < copies/mL <25,000; + + +: >25,000 copies/mL.
  2. bTravel to/from country of origin for foreign patients who travelled abroad in the last month. Travel to countries from endemic areas are indicated in brackets for Italian patients.