|
Method
|
Target region
|
Primer ID
|
Sequence (5′–3′)
|
Product length (bp)
|
Primer position (5′–3′)*
|
|---|
|
Method A
|
ORF1
|
1679
|
CCAYCAGTTYATHAAGGCTCC
|
348
|
36-56
|
| |
1680
|
TACCAVCGCTGRACRTC
| |
383-367
|
| |
1681
|
CTCCTGGCRTYACWACTGC
|
172
|
53-71
|
| |
1682
|
GGRTGRTTCCAIARVACYTC
| |
224-205
|
|
Method B
|
ORF1
|
1829
|
ACATTTGAATTATCTGACATTGTGCA
|
1076
|
3880-3905
|
| |
1828
|
ACACACATCTGAGCTACATTCGTGAG
| |
4955-4930
|
| |
1830
|
GACGTGTCCAGGATCACCTTCTTC
|
559
|
4147-4170
|
| |
1831
|
ACTCACTGCAAAGCACTATCGAAT
| |
4705-4682
|
|
Method C
|
ORF2
|
1722
|
CAAGGHTGGCGYTCKGTTGAGAC
|
506
|
5912-5934
|
| |
1723
|
CCCTTRTCCTGCTGAGCRTTCTC
| |
6417-6395
|
| |
1724
|
GYTCKGTTGAGACCWCBGGBGT
|
457
|
5922-5943
|
| |
1725
|
TTMACWGTCRGCTCGCCATTGGC
| |
6378-6356
|
|
Method D
|
ORF2
|
1837
|
AATTATGCYCAGTAYCGRGTTG
|
731
|
5687-5708
|
| |
1838
|
CCCTTRTCYTGCTGMGCATTCTC
| |
6417-6395
|
| |
1839
|
GTWATGCTYTGCATWCATGGCT
|
348
|
5972-5993
|
| |
1840
|
AGCCGACGAAATCAATTCTGTC
| |
6319-6298
|
|
Method E
|
ORF2/ORF3
|
1847
|
GCRGTGGTTTCTGGGGTGAC
|
164
|
5259-5278
|
| |
1848
|
CTGGGMYTGGTCDCGCCAAG
| |
5422-5403
|
| |
1849
|
GYTGATTCTCAGCCCTTCGC
|
137
|
5282-5301
|
| |
1850
|
GMYTGGTCDCGCCAAGHGGA
| |
5418-5399
|
- *Primer positions are based on GenBank sequence accession no. M73218.
