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Figure 1 | Virology Journal

Figure 1

From: Influenza viral vectors expressing the Brucella OMP16 or L7/L12 proteins as vaccines against B. abortus infection

Figure 1

Genetic stability of the viral constructs after five passages in chicken embryos (CE), as determined by RT-PCR (A) and confirmation of expression of the Brucella proteins L7/L12 or Omp16 by the viral constructs using Western blotting (B). Figure 1A: 1) Flu-NS1-124-Omp16-H5N1; 2) pHW plasmid encoding the NS1-124-Omp16 genes; 3) Flu-NS1-124-Omp16-H1N1; 4) Plasmid NS1-124-Omp16; 5) Flu-NS1-124-L7/L12-H5N1; 6) Plasmid NS1-124- L7/L12; 7) Flu-NS1-124-L7/L12-H1N1; 8) Plasmid NS1-124- L7/L12. Figure 1В: 1) lysed allantoic fluid (AF) of uninfected CE; 2) lysed AF of CE infected with Flu-NS1-124-Omp16-H5N1; 3) lysed AF of CE infected with Flu-NS1-124-Omp16-H1N1; 4) lysed AF of uninfected CE; 5) lysed AF of CE infected with Flu-NS1-124- L7/L12-H5N1; 6) lysed AF of CE infected with Flu-NS1-124- L7/L12-H5N1.

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Virology Journal

ISSN: 1743-422X