Figure 1

Variable virulence of three FV3 strains during tadpole infection. Survival analysis of L. pipiens tadpoles over 41 days of infection with aza-C^r (square), SSME (circle), wt-FV3 (triangle), and Control (diamond). Tadpoles were exposed to FV3 strains for 12 hours, and then along with the virus-contaminated water were transferred to a tank of dechlorinated water for the remainder of the experiment. Survival analysis and failure time analysis was done following the Kaplan & Meier product limit method associated with Chi square and Gehan’s Wilcoxon tests [37].