Figure 3
From: CRTC2 enhances HBV transcription and replication by inducing PGC1α expression
Phosphorylation-defective CRTC2 mutants strongly upregulate HBV biosynthesis. (A) The effect of a phosphorylation-defective mutation of CRTC2 (S171A, S275A, and S171A/S275A) on luciferase activity in pGL-3-ENII-luc transfected Huh-7 cells with or without FSK. (B-D) HBV core protein expression (B), HBV mRNA level (C), and HBV DNA replication intermediates (D) in Huh-7 cells transfected with 1.3× wtHBV plasmid and wild-type or mutated CRTC2 in the presence or absence of FSK. *p < 0.05; **p < 0.01.