Figure 2
From: Cocksfoot mottle virus coat protein is dispensable for the systemic infection
Northern blot analysis showing the RNA silencing suppressor activity of CfMV CP. (A) Northern blot of RNA isolated from the infiltrated patches of N. benthamiana 16c leaves infiltrated as indicated in Figure 1. The upper part shows ethidium bromide staining of rRNA as loading control. The lower part shows the radioactive detection of GFP mRNA. (B) RNA isolated from infiltrated patches of N. benthamiana 16c leaves infiltrated as indicated in Figure 1 were used for detecting GFP siRNAs. The upper part shows ethidium bromide staining of rRNA as loading control. The central part shows the radioactive detection of U6 snRNA also as loading control. The lower part shows the radioactive detection of GFP siRNAs (24 nt, 22 nt and 21 nt long). NC: negative control (non-infiltrated N. benthamiana 16c).