Figure 6

GALV-Nf cytopathology in systemically-infected N. benthamiana leaves. (A) Epidermal cell filled with virus particles (V) and showing some multivesicular bodies (arrows), one of them enlarged in (B). Virions are distributed throughout the cytoplasm, or aggregated in a large crystal (C), or enclosed in membrane-bound enclaves (D). A plastid, enlarged in (E), shows thylakoid disorganization and vesiculation. Similar chloroplast alterations are visible also in parenchyma mesophyll cells (F) and, in some instances, vesicles similar to those forming the multivesicular bodies are observed (arrows). In cells at a late stage of infection, the chloroplast structure appears completely disrupted (G) and electron-dense tubular structures (T) are visible in the stroma. These structures are sometimes present also in mitochondria (H). N, nucleus; P, peroxisome; L, lipid globule. Black bars = 200 nm; white bars = 400 nm.