Identification of an env-defective HIV-1 mutant capable of spontaneous reversion to a wild-type phenotype in certain T-cell lines

Table 3 Sequences of reverted G367R env around CD4 binding site

	HIV Env	365	366	367	368	369	370
HXB2D	A AGA GAA CAA TTT GGA AAT AAT AAA ACA ATA ATC TTT AAG CAA TCC	TCA	GGA	GGG	GAC	CCA	GAA	ATT GTA CGC
G367R	A AGA GAA CAA TTT GGA AAT AAT AAA ACA ATA ATC TTT AAG CAA TCC	TCA	GGA	A GG	GAC	CCA	GAA	ATT GTA CGC
R1-MT2	A AGA GAA CAA TTT GGA AAT AAT AAA ACA ATA ATC TTT AAG CAA TCC	TCA	GGA	GGG	GAC	CCA	GAA	ATT GTA CGC
R2-MT2	A AGA GAA CAA TTT GGA AAT AAT AAA ACA ATA ATC TTT AAG CAA TCC	TCA	GGA	GGG	GAC	CCA	GAA	ATT GTA CGC
R3-MT2	A AGA GAA CAA TTT GGA AAT AAT AAA ACA ATA ATC TTT AAG CAA TCC	TCA	GGA	GGG	GAC	CCA	GAA	ATT GTA CGC
R1-SupT1	A AGA GAA CAA TTT GGA AAT AAT AAA ACA ATA ATC TTT AAG CAA TCC	TCA	GGA	GGG	GAC	CCA	GAA	ATT GTA CGC
R2-SupT1	A AGA GAA CAA TTT GGA AAT AAT AAA ACA ATA ATC TTT AAG CAA TCC	TCA	GGA	GGG	GAC	CCA	GAA	ATT GTA CGC

HXB2D represents wt, G367R represents the mutant plasmid, R1-3 represent the reverted viruses recovered, respectively, from G367R + VSV-G infected MT2 or SupT1 cells when seeded at cell numbers of 10⁵, 10⁴ or 10².

ISSN: 1743-422X