Figure 10

Inhibition of the Akt/p70S6K/eIF4B signaling pathway in cells expressing CyPrP^EGFP. Time-course analysis of the normalized absolute levels of phosphorylated Akt (P-S473), mTOR (P-S2448), p70S6K (P-T389), and eIF4B (P-S422) in N2a cells expressing CyPrP^EGFP for 12, 24, or 48 h; individual data and mean (p-Akt, square; p-mTOR, circle; p-p70S6K, triangle; p-eIF4B, inverted triangle). Due to limited sample, the levels of p-Akt at 12 h were only measured in two biological repeats. The levels of all others were evaluated in three independent biological repeats. Differences in changes in phosphorylation levels in cells expressing CyPrP^EGFP and EGFP was analyzed by replicates test for lack-of-fit. *, P < 0.05; **, P < 0.01; ***, P < 0.001.