Inhibition of the Akt/p70S6K/eIF4B signaling pathway in cells expressing CyPrPEGFP. Time-course analysis of the normalized absolute levels of phosphorylated Akt (P-S473), mTOR (P-S2448), p70S6K (P-T389), and eIF4B (P-S422) in N2a cells expressing CyPrPEGFP for 12, 24, or 48 h; individual data and mean (p-Akt, square; p-mTOR, circle; p-p70S6K, triangle; p-eIF4B, inverted triangle). Due to limited sample, the levels of p-Akt at 12 h were only measured in two biological repeats. The levels of all others were evaluated in three independent biological repeats. Differences in changes in phosphorylation levels in cells expressing CyPrPEGFP and EGFP was analyzed by replicates test for lack-of-fit. *, P < 0.05; **, P < 0.01; ***, P < 0.001.