Table 4 Nucleotide sequences of the designed primers
PCR | Genomic region | Sequence of primers |
|---|---|---|
Nested | 5′UTR | SP1: 5′CTGTGAGGAACTACTGTCTT 3′ |
ASPI : 5′ATACTCGAGGTGCACGGTCTACGAGACCT 3′ | ||
SP2: 5′TTCACGCAGAAAGCGTCTAG 3′ | ||
ASP2: 5′CACTCTCGAGCACCCTATCAGGCAGT 3′ | ||
Nested | Core | SP1: 5′ ACTGCCTGATAGGGTGCTTGC 3′ |
ASP1: 5′ ATGTACCCCATGAGGTCGGC 3′ | ||
SP2: 5′ AGGTCTCGTAGACCGTGCAC 3′ | ||
ASP2: 5′ CACGTAAGGGTATCGATGAC 3′ | ||
Semi nested | NS5B | SP1: 5′ GCCTTCACGGAGGCTATGAC 3′ |
ASP1: 5′ GGCACCCAAGCTTTCTGAG 3′ | ||
ASP2: 5′ ACACGCTGTGATAAATGTC 3′ |