Figure 6

The effects of knockdown of HSP90 on PRRSV infection. (A) MARC-145 cells were transfected with different concentrations of siRNAs for 24 hours and subsequently infected with PRRSV. Cells were harvested at 24 h.p.i for western blotting analysis. The relative protein level of viral N was quantified by measuring band intensities and normalized with respect to the amount of β-actin. Meanwhile, (B) the culture supernatants were collected and viral titers were determined by calculating CCID₅₀. Data are mean ± SD, n = 3, and significant differences compared with control are denoted by ^*(P < 0.05).