The influences of furin suppression on the cellular expression of HBcAg and pre-HBe. HepG2.2.15 cells were regularly cultivated and treated with or without furin inhibitors (20 μmol/L CMK and 100 μmol/L D6R), or transfected with empty pIRES2-EGFP vector (pIRES2) or furin inhibitory prosegment-expressing recombinant vector (pfurin-PS). HBcAg (HBc, 21 kDa), pre-HBe (22 kDa) and HBeAg (HBe, 17 ~ 20 kDa) in cytosol and non-cytosol were detected using Western blot analysis. A: CMK inhibited HBeAg maturation in non-cytosol and accumulated HBcAg and pre-HBe in cytosol. B: D6R and the expression of furin inhibitory prosegment did not accumulate cytosolic HBcAg and pre-HBe. C: The turnover rates of both HBcAg and pre-HBe in cytosol were down-regulated by CMK when new protein synthesis was halted by cycloheximide (CHX).