Figure 5

CREB is expressed to higher levels in scrapie- than in mock-infected mice at 70 and 90 dpi. Targeted secondary analyses of the NMDAR-regulated CaMK4β signaling pathway in brainstem-cerebellum, subcortical and cortical regions of scrapie-infected mice at 70, 90, 110, 130 dpi or at terminal stage of disease (TER; 155–190 dpi). (A) Expression levels of p38γ, Lyn, RSK1, CaMK4β, nNOS, CREB, and PSD-95 in the brainstem-cerebellums of each of the three scrapie-infected mice at each time point, normalized to those in the mock-infected mice, shown by color bars. The proteins in dashed lines were not analyzed. (B) Levels of CaMK4β and CREB in all macrodissected regions, normalized to those in the mock-infected mice, shown as time series. Mean ± SD; n = 3. Error bars on the x-axes, range in time of onset of terminal disease. The differences in the expression levels in scrapie- versus mock-infected mice were analyzed by two-tailed paired ratio t-test. *P < 0.05.