Figure 1From: An efficient genome sequencing method for equine influenza [H3N8] virus reveals a new polymorphism in the PA-X proteinSchematic representation of PCR primer design for genome sequencing EIV. Primers were tagged with M13 forward or reverse sequences for use in the sequencing stage. Segments 1–4 (PB2, PB1, PA and HA) were divided into four sections, segments 5 & 6 (NP and NA) into three sections and segments 7 & 8 (M and NS) into two sections, each of approximately 400–600 nucleotides in length.Back to article page