Figure 2
From: Antiviral potency and functional analysis of tetherin orthologues encoded by horse and donkey
Post-translational modification and subcellular localization of two equine tetherin orthologues. (A) The expression plasmids for human, horse or donkey tetherin homologues (1 μg) were transfected into HEK 293 T cells. Intracellular expression of tetherin was analyzed by Western blotting using anti-HA antibody. (B) 293 T cells were transfected with the expression plasmids for donkey, horse or human tetherin. The cell lysates were treated with (+) or without (−) PNGase Ffor de-N-glycosylated reaction, and then detected with anti-HA antibody by Western blotting. (C) Three tetherin homologues expressed in HEK293T and Hela cells were stained with an anti-HA antibody (green). The cell nuclei were stained with DAPI stain (blue).