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Figure 2 | Virology Journal

Figure 2

From: Chitosan microparticles loaded with yeast-derived PCV2 virus-like particles elicit antigen-specific cellular immune response in mice after oral administration

Figure 2

The porcine circovirus type 2 (PCV2) Cap protein encapsulated in chitosan microparticles. The PCV2 Cap protein was analysed by (A) immunofluorescence microscopy (magnification, ×100), (B) Western blotting, and (C) Dot blot analysis with an anti-PCV2 monoclonal antibody. For the Western and Dot blots, microparticles were denatured by heating to 90°C for 15 min under acidic conditions. The PCV2 Cap protein contained in each formulation was then detected with a PCV2-specific antibody (Jeno Biotech Inc). PCV2 Cap protein was delivered from purified LMW chitosan-derived microparticles (dots A and C) and from non-purified LMW chitosan-derived microparticles (dots B and D).

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Virology Journal

ISSN: 1743-422X