Phylogenetic relationships and pathogenicity variation of two Newcastle disease viruses isolated from domestic ducks in Southern China

Table 7 Replication in geese of the NDV viruses after inoculated intranasally ^a

Virus replication on 3 DPI(log₁₀EID₅₀/g)^bin:	Strains
Virus replication on 3 DPI(log₁₀EID₅₀/g)^bin:	SS-10	NH-10
Heart	ND^c	ND
Liver	2.08 ± 1.01	2.17 ± 0.95
Spleen	5.58 ± 1.01	5.75 ± 0.50
Lung	3.83 ± 0.38	3.08 ± 0.58
Kidney	3.5 ± 1.75	3.00 ± 1.39
Brain	ND	ND
Trachea	1.92 ± 0.72	1.92 ± 0.72
Small intestine	4.08 ± 0.58	3.00 ± 1.39
Bursa of fabricius	4.00 ± 0.66	4.50 ± 0.75
Thymus	4.92 ± 0.52	5.33 ± 0.52
Pancreas	2.58 ± 1.01	1.92 ± 0.28
Cecal tonsils	4.08 ± 0.58	4.83 ± 0.38

^aTwo-week-old geese were inoculated intranasally (i.n.) with 10⁶ EID₅₀ of each virus in a 0.2 ml volume; three ducks in each group were euthanized on 3 DPI, and virus titer was determined in samples of heart, liver, spleen, lung, kidney, brain, trachea, Small intestine, bursa of fabricius, Thymus, Pancreas and cecal tonsils in eggs [17].
^bFor statistical analysis, a value of 1.5 was assigned if the virus was not detected from the undiluted sample in three embryonated hen eggs. Virus titers are expressed as means ± standard deviation in log₁₀EID₅₀/g of tissue.
^cNo detected.

ISSN: 1743-422X