Development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus

Table 1 Replication of recombinant RSV in the upper and lower respiratory tract of BALB/c mice

Days collected^b	Virus^a	Lung	Nose
Days collected^b	Virus^a	Mean titer ± SD^c	Mean titer ± SD^c
		(log₁₀PFU g⁻¹)	(log₁₀PFU ml⁻¹)
3	RSV-Long	4.52 ± 0.29	3.62 ± 0.52
	rLong	3.20 ± 0.18	2.50 ± 0.12
	rLong-∆G-EGFP	2.76 ± 0.36	<1.98
5	RSV-Long	4.42 ± 0.60	3.48 ± 0.25
	rLong	2.86 ± 0.32	2.05 ± 0.08
	rLong-∆G-EGFP	<2.68	<1.98
7	RSV-Long	2.95 ± 0.224	2.65 ± 0.15
7	rLong	<2.68	<1.98
	rLong-∆G-EGFP	<2.68	<1.98

^aBALB/c mice were inoculated with 10⁵ PFU of (recombinant) virus. ^bLungs and nasal washes were collected, and virus titers were determined on the indicated day: 3, 5, or 7. ^cThe lower limits of detection for virus in the lungs and nose were 2.68 log₁₀ PFU g⁻¹ and 1.98 log₁₀ PFU ml⁻¹, respectively. Each group consisted of six animals.

ISSN: 1743-422X