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Figure 2 | Virology Journal

Figure 2

From: Development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus

Figure 2

Identification of recovered recombinant viruses by fluorescent assay and plaque assay. (A) the successfully recovery of rLong was confirmed through indirect immune fluorescent assay by immune-staining with an anti-RSV G1 polyclonal antibody targeting G protein epitopes as the first antibody. (B) the recovery of rLong-∆G-EGFP was evaluated by observating under fluorescent microscope. (C) the negative control was Hep-2 cells without infected by viruses. (D-F) Plaque morphology for wild-type RSV long virus, rLong and rLong-∆G-EGFP respectively.

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Virology Journal

ISSN: 1743-422X