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Table 1 Primers used to amplify and sequence the complete BATV S, M, and L segments

From: Isolation and complete nucleotide sequence of a Batai virus strain in Inner Mongolia, China

Segment primer Sequence (5′ to 3′) Amplicon size (nt)
S segment primer   
BATAS1F 5′-GAATTCAATGATGTCGCTGCT-3′ 818
BATAS1R 5′-CAATTTGCTGTGCTCTTTCTG-3′
3′S-Inner Primer 5′-CTAGCAAAGTTTGGCATCAGC-3′  
3′S-Outer Primer 5′-GGGTGGAAGAAGACAAATGTG-3′
5′S-Inner Primer 5′-GGGTCAAAAGTACTGCTGGTG-3′
5′S-Outer Primer 5′-GTGTAGATACGCTTAAAGTTA-3′
M segment primer   
BATAM1F 5′-GATGTTGCTACTTCTTGTCTT-3′ 2013
BATAM1R 5′-AGTTTTGTAATACCAGTTGTG-3′
BATAM2F 5′-CAACTGAGTCACTCTTTTATG-3′ 1973
BATAM2R 5′-CATAATCTTGTTTTGTGGAGG-3′
BATAM3F 5′-CTGCAGTTTTAAGATTGTTTC-3′ 480
BATAM3R 5′-TGTTTTATTTCCTGTAGGTAC-3′
3′M-Inner Primer 5′-GTACCTACAGGAAATAAAACA-3′  
3′M-Outer Primer 5′-TGATGTTTAAACTTAGAGATG-3′
5′M-Inner Primer 5′-GAGGCAAAATTCTGATATAGC-3
5′M-Outer Primer 5′-ATTTTTTTCATATATTATTTC-3′  
L segment primer   
BATAL1F 5′-AAAAATGGATGATCAGATGTA-3′ 2025
BATAL1R 5′-TTCTCTGCTATATAATCTTTG-3′
BATAL2F 5′-TCGATATATGATAATGAATTC-3′ 2021
BATAL2R 5′-CTAAACCAACTAAAGCTATCA-3′
BATAL3F 5′-TTAAGAGTCGTCATGATATAC-3′ 1952
BATAL3R 5′-TGATAATACAACAGGACAGAC-3′
BATAL4F 5′-TAAAAAGAAATGAAGAAGGAC-3′ 945
BATAL4R 5′-CTTAGAAAAAGGTGAACATGG-3′
3′L-Inner Primer 5′-CAACTGGACCAAGATGCTAAA-3′  
3′L-Outer Primer 5′-CTGCCTTCTACATAAATACAG-3′  
5′L-Inner Primer 5′-GTTCCAACTGATACTTTATAG-3′  
5′L-Outer Primer 5′-GTATTTTTTATAAGTAATCTC-3′  
P1 5′-ATAGAATCAGCAATAGCAAGC-3′ 550
P2 5′-ATGATGATCTGTAACCTCTAA-3′