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Table 3 PCR efficiency after manual and automated extraction

From: Development and validation of rapid magnetic particle based extraction protocols

Target Material % PCR efficiency
Manual EZ1 BS 15 KF Duo
SBV Blood 99.6 97.0 86.1 101.2
Serum 97.8 121.5 87.1 112.7
BVDV Blood 112.4 99.3 88.8 84.2
Serum 95.0 98.2 92.8 110.8
  1. 10-fold dilution series of SBV- and BVDV-positive blood and serum samples which were extracted in duplicate; PCR efficiencies of the respective RNA extracts were calculated from the slope of the standard curves.