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Table 3 PCR efficiency after manual and automated extraction

From: Development and validation of rapid magnetic particle based extraction protocols

Target

Material

% PCR efficiency

Manual

EZ1

BS 15

KF Duo

SBV

Blood

99.6

97.0

86.1

101.2

Serum

97.8

121.5

87.1

112.7

BVDV

Blood

112.4

99.3

88.8

84.2

Serum

95.0

98.2

92.8

110.8

  1. 10-fold dilution series of SBV- and BVDV-positive blood and serum samples which were extracted in duplicate; PCR efficiencies of the respective RNA extracts were calculated from the slope of the standard curves.
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Virology Journal

ISSN: 1743-422X