Figure 1
From: Development and validation of rapid magnetic particle based extraction protocols
Analytical sensitivity and linear dynamic range of manual and rapid automated extraction methods. Dilution series of serum samples positive for SBV-RNA (A) or BVDV-RNA (B) as well as dilution series of EDTA-blood samples positive for SBV-RNA (C) or BVDV-RNA (D) were used to evaluate the extraction efficiency of the rapid extraction protocols in comparison to the standard manual extraction protocol. Each dilution step was extracted in duplicate and the extracted RNA from each panel was tested in the same RT-qPCR run. R2 values as defined by regression analysis are indicated for each method.