3 | 2 | 1 | Construct |
---|---|---|---|
mut | Wt | wt | K |
mut | Wt | mut | L |
mut | Mut | wt | M |
wt | Mut | wt | N |
wt | Mut | mut | O |
wt | Wt | mut | P |
Construct | Oligos used (Oligo 2 is the same for all constructs) | ||
K | Oligo 1: TCGGAGGACAGTACTCCGACCCGGTCGAAGGGATTCGTGGCCTGGGCGGGACTGGGGAGTGGCGAGCCCTCAGATCCTGCATATAA | ||
Oligo 2: GAGCCCTCAGATCCTGCATATAA GCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATC CGAGC | |||
L | Oligo 1: TCGGAGGACAGTACTCCGACCCGGTCGAAGGGATTCGTGGCCTGGGCGGGACTGGTTAGTGGCGAGCCCTCAGATCCTGCATATAA | ||
M | Oligo 1: TCGGAGGACAGTACTCCGACCCGGTCGAAGGGATTCGTGGCCTGTTCGGGACTGGGGAGTGGCGAGCCCTCAGATCCTGCATATAA | ||
N | Oligo 1: TCGGAGGACAGTACTCCGACCCGGTCGAAGGGAGGCGTGGCCTGTTCGGGACTGGGGAGTGGCGAGCCCTCAGATCCTGCATATAA | ||
O | Oligo 1: TCGGAGGACAGTACTCCGACCCGGTCGAAGGGAGGCGTGGCCTGTTCGGGACTGGTTAGTGGCGAGCCCTCAGATCCTGCATATAA | ||
P | Oligo 1: TCGGAGGACAGTACTCCGACCCGGTCGAAGGGAGGCGTGGCCTGGGCGGGACTGGTTAGTGGCGAGCCCTCAGATCCTGCATATAA |