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Table 1 SpI deletion construct oligos and primers

From: The CD8 Antiviral Factor (CAF) can suppress HIV-1 transcription from the Long Terminal Repeat (LTR) promoter in the absence of elements upstream of the CATATAA box

S No

Name of construct

DNA oligos used

1

Del 1, del 2

5’TCGGAGGACAGTACTCCGACCCGGTCGAAGGGAGGCGTGGCCTGAGCCCTCAGATCCTGCATATAA GC

And

5’GAGCCCTCAGATCCTGCATATAA GCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCCGAGC

2

Del 1

5’TCGGAGGACAGTACTCCGACCCGGTCGAAGGGAGGCGTGGCCTGGGCGGGACTGAGCCCTCAGATCCTGCATATAA GC

And

5’GAGCCCTCAGATCCTGCATATAA GCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCCGAGC

3

Del 2

5’TCGGAGGACAGTACTCCGACCCGGTCGAAGGGAGGCGTGGCCTTGGGGAGTGGCGAGCCCTCAGATCCTGCATATAA

And

  

5’GAGCCCTCAGATCCTGCATATAA GCAGCTGCTTTTTGCCTGTACTGGGTCTCTCTGGTTAGACCAGATCCGAGC

  1. PCR primers used for amplifying all the constructs: Fwd TGTGAAGCTTTCGGAGGACAGTACTC, Rev: TGTGGAGCTCGGATCTGGTCTAAC.
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Virology Journal

ISSN: 1743-422X