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Table 3 Discordant results obtained during the clinical evaluation

From: Detection of circulating norovirus genotypes: hitting a moving target

No.

RT-PCR results

Discordant analysis

Interpretation

Comments

Real-time RT-PCR

EP-JV

EP-SR

Result

Genotype

5

Neg.

Neg.

Pos.

Neg.

N/A

FP (EP-SR)

Non-specific amplification

3

Neg.

Pos.

Neg.

Neg.

N/A

FP (EP-JV)

Non-specific amplification

8

Pos.

Neg.

Neg.

Pos.

GII.7

FN (EP-JV and EP-SR)

High viral loads (5.28 to 7.78 log10 copies/ml)

3

Pos.

Pos.

Neg.

Pos.

GI.6

FN (EP-SR)

High viral loads (5.29 to 5.74 log10 copies/ml)

1

Pos.

Pos.

Neg.

Pos.

GI.3

FN (EP-SR)

Low viral load (2.47 log10 copies/ml)

1

Pos.

Neg.

Pos.

Pos.

GII.15

FN (EP-JV)

High viral load (6.33 log10 copies/ml)

  1. Abbreviations: FN false negative, FP false positive, N/A not applicable, Neg. Negative, No. number, Pos. positive.