Figure 6

The results of HSV-1 gC immunoperoxidase (IPS) staining (blue bars) and HSV-1 titer (black bars). The graph shows HMK cells, infected with HSV-1 at MOI shown and irradiated 24 hours post infection. The measurements were done from medium samples taken from the cultures at times of fixation for IPS at designated hours post irradiation. HSV-1 titer was measured using standard plaque titration assays on b-Vero cells. The numbers represent the averages of a minimum of quadruplicate cultures +/- SEM. The values for IPS staining were averaged from the results of two independent observers. The photomicrographs, taken at 400 × from a random location on the culture, present HMK cells infected with 0.0001MOI HSV-1 and irradiated (A) or nonirradiated (B) and fixed for IPS at 144 hours post irradiation. No significant differences in IPS staining or virus production were found when irradiated- and nonirradiated cultures infected with the same MOI were compared (Mann–Whitney U-test, p value range from p = 1 to p = 0.121).