Figure 3
From: Small molecules that inhibit Vif-induced degradation of APOBEC3G
Candidate molecules impaired virus infectivity in the presence of both APOBEC3G and Vif. VSV-G pseudo-typed luciferase-reporter virus was produced in the presence or absence of co-transfection of APOBEC3G expression vector. Before harvesting virus-containing supernatant, 10 μM MM-1 or MM-2 were added to culture medium. Harvested supernatant was challenged to fresh 293 T cells, and luciferase activity of lysates was measured after two days. Samples of virus and producer cells were also analyzed by immunoblotting. APOBEC3G levels in the virus particles were recovered by treatment of the compounds.