Figure 2
From: Small molecules that inhibit Vif-induced degradation of APOBEC3G
Detailed analyses of the candidate molecules. A. Titration of the two compounds. Treatment of MM-1 or MM2 recovered fluorescence intensity and protein levels of EGFP-fused APOBEC3G in a dose-dependent manner. 293 T cells were co-transfected with EGFP-APOBEC3G and Vif expression vectors and treated with different concentrations of MM-1 or MM-2. Fluorescence intensity was measured and that of DMSO-treated cells without Vif expression was normalized to 100%. Cell lysates were also analyzed by immunoblotting (panels). B. Vif dependency of the compounds. 293 T cells were transfected with EGFP-APOBEC3G expression vector in the absence or presence of co-transfection of Vif expression vector, and treated with 10 μM MM-1 or MM-2 for 24 hours. Cells were analyzed similarly to A, average and standard error of two independent experiments are shown in histogram.