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Figure 4 | Virology Journal

Figure 4

From: Identification and validation of a novel microRNA-like molecule derived from a cytoplasmic RNA virus antigenome by bioinformatics and experimental approaches

Figure 4

Knockdown of Dicer reduces the expression of HAV antigenome-encoded hav-miR-N1-3p. (A) Dicer mRNA was knocked down by RNAi and confirmed by qRT-PCR. (B) Dicer protein was knocked down by RNAi and confirmed by Western blot. Actin was used as a loading control. (C) Expression difference analysis of HAV antigenome-encoded hav-miR-N1-3p between Dicer-silencing and negative siRNA-transfected cells. HAV antigenome-encoded hav-miR-N1-3p expression changes were calculated relatively to U6 snRNA, and cellular miR-154 was used as a positive control. Significant difference in expression levels were detected by Student’s t-test (*P < 0.05 and **P < 0.01).

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Virology Journal

ISSN: 1743-422X