Knockdown of Dicer reduces the expression of HAV antigenome-encoded hav-miR-N1-3p. (A) Dicer mRNA was knocked down by RNAi and confirmed by qRT-PCR. (B) Dicer protein was knocked down by RNAi and confirmed by Western blot. Actin was used as a loading control. (C) Expression difference analysis of HAV antigenome-encoded hav-miR-N1-3p between Dicer-silencing and negative siRNA-transfected cells. HAV antigenome-encoded hav-miR-N1-3p expression changes were calculated relatively to U6 snRNA, and cellular miR-154 was used as a positive control. Significant difference in expression levels were detected by Student’s t-test (*P < 0.05 and **P < 0.01).